cd105 protein  (Sino Biological)

Journal: Physiological Reports

Article Title: Dapagliflozin prevents ERK activation and SGLT2‐dependent endoglin upregulation in a mechanically provoked cardiac injury model

doi: 10.14814/phy2.15990

Figure Lengend Snippet: TGF‐β1 and CD105 demonstrated as CAD biomarkers. (a, b) Bar graphs representing serum levels of TGF‐β1 and CD105 with different CAD severity. (c, d) Receiver operating characteristic (ROC) curve and area under the curve (AUC) for TGF‐β1 and CD105 (AUC = 0.701 and 0.731 respectively). Diagonal line indicates zero predictive value for the model. Data were analyzed with the Mann–Whitney U test. Correlations were assessed using Spearman's rank correlation moment correlation coefficient.

Article Snippet: To analyze CD105 and TGF‐β level, the human CD105 ELISA kit (Boster Biological Technology, CatEK0644) and human TGF‐β assay ELISA kit (Boster Biological Technology, CatEK0513) were used to detect CD105 and TGF‐β level, respectively, in the collected serum.

Techniques: MANN-WHITNEY

Journal: Physiological Reports

Article Title: Dapagliflozin prevents ERK activation and SGLT2‐dependent endoglin upregulation in a mechanically provoked cardiac injury model

doi: 10.14814/phy2.15990

Figure Lengend Snippet: TGF‐β1 and CD105 demonstrated as CAD biomarkers. (a, b) Bar graphs representing serum levels of TGF‐β1 and CD105 with different CAD severity. (c, d) Receiver operating characteristic (ROC) curve and area under the curve (AUC) for TGF‐β1 and CD105 (AUC = 0.701 and 0.731 respectively). Diagonal line indicates zero predictive value for the model. Data were analyzed with the Mann–Whitney U test. Correlations were assessed using Spearman's rank correlation moment correlation coefficient.

Article Snippet: To analyze CD105 and TGF‐β level, the human CD105 ELISA kit (Boster Biological Technology, CatEK0644) and human TGF‐β assay ELISA kit (Boster Biological Technology, CatEK0513) were used to detect CD105 and TGF‐β level, respectively, in the collected serum.

Techniques: MANN-WHITNEY

Journal: Physiological Reports

Article Title: Dapagliflozin prevents ERK activation and SGLT2‐dependent endoglin upregulation in a mechanically provoked cardiac injury model

doi: 10.14814/phy2.15990

Figure Lengend Snippet: Spearman's rank correlation coefficient for serum TGF‐β1 and CD105, and the different parameters assigned in this study.

Article Snippet: To analyze CD105 and TGF‐β level, the human CD105 ELISA kit (Boster Biological Technology, CatEK0644) and human TGF‐β assay ELISA kit (Boster Biological Technology, CatEK0513) were used to detect CD105 and TGF‐β level, respectively, in the collected serum.

Techniques:

Journal: Physiological Reports

Article Title: Dapagliflozin prevents ERK activation and SGLT2‐dependent endoglin upregulation in a mechanically provoked cardiac injury model

doi: 10.14814/phy2.15990

Figure Lengend Snippet: Troponin I and CD105 increased in cardiomyocytes after mechanical stretch. (a, b) Representative images showing troponin I and CD105‐positive cells in human cardiomyocytes after 24 and 48 h cyclic stretch. Cells were counterstained in 4,6‐diamidino‐2‐phenylindole. Scale bar = 50 μm. (c, d) Bar graphs representing troponin I and CD105 fluorescence intensity. Data represented at least three independent experiments in median (interquartile range). Data were analyzed with the Mann–Whitney U test.

Article Snippet: To analyze CD105 and TGF‐β level, the human CD105 ELISA kit (Boster Biological Technology, CatEK0644) and human TGF‐β assay ELISA kit (Boster Biological Technology, CatEK0513) were used to detect CD105 and TGF‐β level, respectively, in the collected serum.

Techniques: Fluorescence, MANN-WHITNEY

Journal: Physiological Reports

Article Title: Dapagliflozin prevents ERK activation and SGLT2‐dependent endoglin upregulation in a mechanically provoked cardiac injury model

doi: 10.14814/phy2.15990

Figure Lengend Snippet: SGLT2 inhibitor decreased troponin I and CD105 protein expressions. (a) Representative images showing green fluorescence‐labeled troponin I and CD105‐positive cells in cardiomyocytes. Cardiomyocytes were treated with DAPA (SGLT2 inhibitor) or PD98059 (ERK inhibitor), followed by 25%, 24 h cyclic stretch. Scale bar = 50 μm. (b, c) Bar graphs showing troponin I and CD105 fluorescence intensity in the cardiomyocytes. (d) Correlation of troponin I and CD105 with cyclic‐stretch‐induced mechanical overloading was assessed. Data represented at least three independent experiments in median (interquartile range). Data were analyzed with the Mann–Whitney U test. Correlations were assessed using Spearman's rank correlation moment correlation coefficient.

Article Snippet: To analyze CD105 and TGF‐β level, the human CD105 ELISA kit (Boster Biological Technology, CatEK0644) and human TGF‐β assay ELISA kit (Boster Biological Technology, CatEK0513) were used to detect CD105 and TGF‐β level, respectively, in the collected serum.

Techniques: Fluorescence, Labeling, MANN-WHITNEY

Journal: Physiological Reports

Article Title: Dapagliflozin prevents ERK activation and SGLT2‐dependent endoglin upregulation in a mechanically provoked cardiac injury model

doi: 10.14814/phy2.15990

Figure Lengend Snippet: Proposed mechanism for Dapagliflozin on troponin I, endoglin (CD105) and ERK activation in the mechanically injured cardiac cell model. Our model depicts the upregulation of troponin I and endoglin (CD105) after mechanically triggered cardiac injury. (a) Cardiomyocytes were seeded on stretchable PDMS membrane, subjected to cyclic stretch at 5% and 25% elongation. After cyclic stretch, troponin I and CD105 were increased, triggering downstream p‐ERK to enter cell nucleus, potentially altered gene transcription that promoted upregulation of p‐eNOS. The accumulated p‐ERK nuclear translocation and p‐eNOS activation led to an imbalanced regulation of sodium/calcium ions. Finally, the cardiomyocytes were overwhelmed by loss of sodium/calcium ions homeostasis, renting the cells susceptible to CVD‐related risk factor. (b) Dapagliflozin (DAPA), the inhibitor having higher affinity for SGLT2, is able to prevent the characteristics of the mechanically provoked biomarkers for cardiac injury. As a result, SGLT2 and/or SGLT1 is crucial in maintaining the normal function of cardiomyocytes.

Article Snippet: To analyze CD105 and TGF‐β level, the human CD105 ELISA kit (Boster Biological Technology, CatEK0644) and human TGF‐β assay ELISA kit (Boster Biological Technology, CatEK0513) were used to detect CD105 and TGF‐β level, respectively, in the collected serum.

Techniques: Activation Assay, Membrane, Translocation Assay